In this article, we report an imaging method, termed Fourier ptychographicmicroscopy (FPM), which iteratively stitches together a number of variablyilluminated, low-resolution intensity images in Fourier space to produce awide-field, high-resolution complex sample image. By adopting a wavefrontcorrection strategy, the FPM method can also correct for aberrations anddigitally extend a microscope's depth-of-focus beyond the physical limitationsof its optics. As a demonstration, we built a microscope prototype with aresolution of 0.78 um, a field-of-view of ~120 mm2, and a resolution-invariantdepth-of-focus of 0.3 mm (characterized at 632 nm). Gigapixel colour images ofhistology slides verify FPM's successful operation. The reported imagingprocedure transforms the general challenge of high-throughput, high-resolutionmicroscopy from one that is coupled to the physical limitations of the system'soptics to one that is solvable through computation.
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